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"elnifro" - 1 õppematerjal

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Liha töötlemine

fore, routine methodologies for GMO range of food matrices (Yates 1999). detection adapted to multiple analyses are Furthermore, DNA content per mass must be convenient. Multiplex PCR requires several considered because it fluctuates among dif- primers that lead to amplification of unique ferent cultivars of the same species due to the DNA regions under a single reaction (Atlas variation of the DNA content of endosperm, and Bej 1994; Elnifro et al. 2000; Wittwer et embryo, and teguments of kernels (Trifa and al. 2001). This approach is cheaper and less Zhang 2004). Currently, only high-quality labor intensive, saving time and effort. DNA samples purified from certified raw However, the use of many PCR primers in a materials or plasmids are being used as refer- single tube can cause some problems, such ence controls to determine the ratio of trans-

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