Capillary electrophoresis i.k.
More often, to delete adsorption products on surface, conditioning by alkalis is
applied. Typical conditioning cycle comprises washing with 1 M NaOH, then 0.1 M NaOH,
and finally filling the buffer. Before each analysis are performed only the last two steps.
Another method consists in washing with a strong acid or with organic solvents such as
methanol, dimethylsulfoxide or detergents.
Thermal regulation
A strong dependence of the input volume and the migration time of the viscosity necessitates
thermostation with accuracy ± 0.1 °C using a water or air bath. Although thermostating in
liquid is more efficient, air flow of about 10 m / s are suitable for heat dissipation in HECE to
5 W / m
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