Liha töötlemine
racy, even at species level. The most fre- sample is achieved by using specific probes
quently applied and most reliable technique that allow spatial distribution studies to take
is sequencing of the 16S-rRNA gene. This place.
technique has been applied either in combi- The microorganisms that are most fre-
nation with other techniques, such as SDS- quently encountered are Lactobacillus curva-
PAGE of whole cell proteins, RAPD-PCR, tus, Lb. plantarum, Lb. sakei, Staphylococcus
or PFGE, or directly to the isolated microor- carnosus, St. saprophyticus, and St. xylosus
ganism. Although this approach provides (Table 9.1). These microorganisms seem to
accurate identification at strain level, an be autochthonous in this ecosystem and have
equally important part of the microbiota, the capacity to prevail during fermentation.
annaabi.ee 
