"ctgaatgggggccccccccctcagggtcgacggtatcgataagcttggatatcgaattcc" - 1 õppematerjal

Molekulaarbioloogia protokoll

(1) Saadud DNA järjestuse editeerimine, vastavalt saadud elektroforegrammile, kasutades programme MS Word, DNAMAN, Chromas, jt. plasmiidii osa lahutamine. (2) DNA järjestuse võrdlemine andmepankades olevate tuntud järjestustega kasutades BLASTN, MEGABLAST, BLAST 2 sequences jt programme (http://www.ncbi.nlm.nih.gov/) (3) "CpG island"i identifitseerimine inimese kromosoomis (BLAT programm, http://genome.ucsc.edu/cgi-bin/hgBlat) Proov nr.8. CTGAATGGGGGCCCCCCCCCTCAGGGTCGACGGTATCGATAAGCTTGGATATCGAATTCC TGCAGCCCGGGGGATCCACTAGATTTAGAGCGGCCGCCACCGCGGCAGGCCGACGGCCCC ACGGAGGGACATTGAAGGGGAAGTGGAGGAGAAGAGCAGGGGCGACCCGGAGGGGTGGC TGGGCCGGGGCGGGCAAGACAGTCTCAGGGCCCGGCAGCCGCCCGGGGTTGGCTAATTTCT AGTTGTTGCCCCGCTCAGGGCGGAGCGCCGAGGGGAAGGAGGCCGCGGGGCCGCGCGAAG GCTCGGGGTAGCAACGAGGCCCGGAGGGGGCCGAGCAGGGCGCCCCGCCCCGAGTCCCCG CACCCGCGCCCGCCGGGTCCGCGTTCCAGCGCGTGCCTAGCAGTCCGCGCCTGGGGCCGG GCGCTCACCGGACTTCGGAGGGTAGCGATAGGCCGAGTTCGCCTGGATGTCGATGTCCTC CACCCCCGCGATGCGGCGGCTGAGAATGGAGCCCATGGTGCGCCGCGCTGCCGGGCAGAG