Liha töötlemine
Leu-Val-Tyr-AMC, regardless of the pH or to be the predominant isoform (Kaminski and
ionic strength. Similar results were seen Andrade 2001). These enzymes utilize argi-
when using purified myofibrils as the sub- nine as a substrate and catalyze the following
strate. This inhibition was reversible, as reaction: L-arginine+NADPH+O2 forming
addition of reducing agent (DTT) to the oxi- L-citrulline+•NO+NADPH+. NO is important
dized samples restored activity. Oxidation in biological systems, particularly because of
also has been shown to slow the rate of μ- its role as a second messenger. However,
calpain autolysis and could be part of the while NO rapidly diffuses through tissues,
mechanism underlying some of the retarda- NO itself is a relatively short-lived species.
tion of activity (Guttmann et al
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