Liha töötlemine
2006). The proteasome, first
and ionic strength combinations may not isolated in 1980 (Wilk and Orlowski 1980),
provide an accurate estimation of activity is a barrel-shaped polypeptide structure with
because of precipitation of calpain or the active sites in its interior core (Mykles and
alteration of the interaction with substrates. Harie 1995; Attaix et al. 1998, 2001;
These issues have been the topic of much Glickman and Cienchanover 2002). A 20S
debate in the literature (Prates 2002; Goll et latent form is part of the 26S proteasome
al. 2003; Geesink and Veiseth 2009), but the (Attaix et al. 1998, 2001) and can be acti-
prevailing belief is that μ-calpain is the vated by mild denaturing treatments
essential and predominant enzyme responsi- (Yamamoto et al. 2009) such as heat, chemi-
ble for postmortem proteolysis and that com- cals, or high pressure. At least five multipep-
annaabi.ee 
