Liha töötlemine
and one primer of each set was labeled. The based on multiplex assays on the basis of
detection limit was below the EU recommen- SNPlex technology (Applied Biosystems),
dation (0.25%) for each GMO. Xu et al. which allows the simultaneous detection of
(2006) designed three different microarrays, up to 79 SNPs in two panels containing 47
the first for the most used regulatory and 48 probes, respectively (Chaouachi et al.
sequences including the 35S promoter, 35S 2008).
terminator, nos promoter, nos terminator, While the quantification obstacles for the
nptII terminator, and the FMV 35S promoter, microarray approaches are surmounted, its
while the second microarray treated specific practical application in GMO analysis will
gene inserts (soybean, cotton, and rapeseed), probably be restricted to an initial step for