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of polycationic chitosan, which readily inter- acts with proteins, fats, and other anionic Plant-derived essential oil components compounds, chitosan antimicrobial activity is may be active against bacteria but are diffi- less in foods than in vitro (Rhoades and cult to apply in foods due to significant Roller 2000). Chitosan has achieved self- changes in sensory quality (Davidson 2001). affirmed GRAS status (FDA-CFSAN 2004), Seydim and Sarikus (2006) compared the removing regulatory restrictions on its use in antimicrobial activity of oregano, rosemary, some foods. and garlic essential oils in whey protein Studies by Darmadji and Izumimoto isolate films (1.0 to 4.0% wt/vol) against (1994) showed that 1% chitosan addition to E. coli O157:H7, Staphylococcus aureus,

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