Liha töötlemine
These are and Holst-Jensen 2001; Taverniers et al.
based on genes present in both transgenic and 2001; Terry and Harris 2001), for transgenic
nontransgenic varieties of the same plant maize lines, such as MON810 (Holck et al.
species. They must not present genetic modi- 2002; Hernandez et al. 2003a), CBH-351
fications (a SNP was detected in ADH1 gene (Windels et al. 2003), Bt11 (Zimmermann et
affecting quantification; Broothaerts et al. al. 2000), and NK603 (Nielsen et al. 2004).
2008), and must show species-specificity, Table 29.1 summarizes the published GMO
stability among different varieties of the analytical systems based on PCR.
510 Chapter 29
Table 29.3. PCR methods for analysis of endogenous reference genes. cPCR: conventional PCR.
RTi-PCR: real-time PCR. Modified from Hernandez et al. (2005)
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