Liha töötlemine
A 20S
debate in the literature (Prates 2002; Goll et latent form is part of the 26S proteasome
al. 2003; Geesink and Veiseth 2009), but the (Attaix et al. 1998, 2001) and can be acti-
prevailing belief is that μ-calpain is the vated by mild denaturing treatments
essential and predominant enzyme responsi- (Yamamoto et al. 2009) such as heat, chemi-
ble for postmortem proteolysis and that com- cals, or high pressure. At least five multipep-
bined m- and μ-calpain activity may be tidase activities (Mykles and Harie 1995)
responsible for up to 85% of postmortem have been described: trypsin-like, chymo-
meat tenderization (Geesink et al. 2000; trypsin-like, branched-chain amino acid-pre-
Geesink et al. 2006) ferring, small neutral amino acid-preferring,
and peptidylglutamyl peptide hydrolase