Liha töötlemine
size by grinding in homogenizers, such as number by using the C-value (Arumuganathan
blenders, stomacher, polytron, ultra-turrax, and Earle 1991; Bennett and Leitch 2003).
mills, and mortars, which reduce consider- Purity of DNA can be evaluated by assessing
ably the sampling error (Begg et al. 2007). the degree of degradation using agarose
504 Chapter 29
electrophoresis, by means of the ratio of on nested PCR are available in the bibliogra-
UV spectrophotometry at 260/280 nm (desir- phy (see Table 29.1).
ably close to 1.8) and 260/230 (above 1.8–
2.2); and by the determination of the presence
Quantitative PCR
of PCR inhibitors by spiking a control
sample. In certain circumstances, such as the determi-
nation of percentage of transgenic ingredients
annaabi.ee 
