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qualitative (presence/absence) detection, in
2008). This technique is based on the genome which any food laboratory is encouraged to
amplification by multiple displacement participate to test its performance.
amplification (MDA). It is based on the use A common set of criteria for performance
of a specific DNA polymerase, phi29 DNA of GMO detection methods should be evalu-
polymerase, and random hexamer primers ated (Bertheau et al. 2002; Bellocchi et al.
for the replication of genomic DNA in an 2008; Žel et al. 2008). Recommendations for
isothermal reaction at 30°C, leading to the the validation of quantitative PCR methods
synthesis of large amounts of DNA with are presented by Hübner and coworkers
fragments >70 kb in size. (2001), and currently there is an international
standard that defines the PCR performance
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