"approv" - 1 õppematerjal

Mageveekäsna Ephydatia fluviatilis populatsiooni geneetiline analüüs

Moonsund Sea and the Gulf of Riga drainage basin: River Lokuta 10 min. (specimen L), River Pedetsi (specimens Pe1 and Pe2) and River The success of PCR amplifications was initially evaluated by Ko~pu (specimens K1 and K2). The study was approved by The electrophoresis of the products in 1% agarose gel. The amplifi- Estonian Environmental Board. No additional permits or approv- cation products were purified using the JetQuick PCR Purification als were required. The cosmopolitan sponge species Ephydatia Kit (Genomed, Germany). fluviatilis is a representative of the phylogenetically lowest DNA fragments of ,1091 bp obtained by PCR amplification multicellular animals and there are no ethics regulations concern- were directly sequenced using ABI PRISM 3130 Genetic Analyzer ing this species. E