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PCR product by means of plotting fluores- result is an amplification plot. A typical
cence as a function of temperature to generate amplification curve presents three different
a melting curve of the amplicon at the end phases. The initiation phase occurs during
point (Ririe et al. 1997). Another nonspecific the first PCR cycles when the emitted fluo-
detection system is the Sunrise primers rescence cannot be distinguished from the
(AmpliFluor™ system) developed by Intergen baseline. During the exponential or log phase
Co. It uses a universal, energy-transfer hairpin there is an exponential increase in fluores-
primer (UniPrimer™) that emits a fluorescent cence, and finally, during the plateau phase,
signal when unfolded during its incorporation the reagents are exhausted, and no increase
into an amplification product (Nazarenko in fluorescence is observed. Quantification is
et al. 1997)
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