Mageveekäsna Ephydatia fluviatilis populatsiooni geneetiline analüüs
Moonsund Sea and the Gulf of Riga drainage basin: River Lokuta 10 min.
(specimen L), River Pedetsi (specimens Pe1 and Pe2) and River The success of PCR amplifications was initially evaluated by
Ko~pu (specimens K1 and K2). The study was approved by The electrophoresis of the products in 1% agarose gel. The amplifi-
Estonian Environmental Board. No additional permits or approv- cation products were purified using the JetQuick PCR Purification
als were required. The cosmopolitan sponge species Ephydatia Kit (Genomed, Germany).
fluviatilis is a representative of the phylogenetically lowest DNA fragments of ,1091 bp obtained by PCR amplification