Mageveekäsna Ephydatia fluviatilis populatsiooni geneetiline analüüs
In the case of nucleotide substitutions,
short sedimentation and the spicules were eliminated by filtration the peak heights corresponding to the particular nucleotides in the
of the suspension through nylon gauze (50 mm mesh) [9]. The cells same position on the electropherogram were compared as
to be used for DNA extraction were isolated by centrifugation previously described [12]. A novel modelling strategy, the
(5006g, 4uC, 10 min), frozen in liquid nitrogen and stored at proportion model method, was developed for relative quantifica-
220uC. tion of the alleles differing due to indels. This method allows for
Gemmules from specimens K1, K2, L, Pe1, Pe2, Pi, V1 and V2 quantifying the sequences having insertions or deletions within a
annaabi.ee 
