Liha töötlemine
508 Chapter 29
tive first primer extension reaction, using junction regions of DNA from different
tailed primers, and a subsequent transcrip- origins, and flanking regions of the intro-
tion-based amplification, using universal duced construction, which aims to determine
primers. The NAIMA product is directly which GMO is present in the sample, and
ligated to fluorescent dyes labeled 3DNA therefore, if it is authorized or not; and
dendrimers, allowing signal amplification finally, (3) GMO-quantification that targets
and hybridized without further purification the specific gene, or the border sequences,
on an oligonucleotide probe-based microar- and accurately quantifies the percentage of
ray for multiplex detection. Another inge- GMO in the food product as a necessary step
annaabi.ee 
