Mageveekäsna Ephydatia fluviatilis populatsiooni geneetiline analüüs
PCR amplification, cloning and sequencing different runs were normalized, so that the mean of the intensities
The ITS region including ITS1, 5.8S gene and ITS2 was in a sequence would be as close as possible to 1000 units. Then to
amplified using the primers 18Sfw: 59-TAC ACA CCG CCC obtain the expected values, the mean value among clones for a
GTC GCT ACT A and 28S59rev: 59-GAC GTG CCT TTC particular nucleotide in each position was found. The normaliza-
CAG GTC AAC TT. The primers were designed to include the tion was needed also because different primers were used for
invariant sequence before the heterogenic positions in the sequencing the clones, so any particular nucleotide in the rDNA
amplicon
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