Mageveekäsna Ephydatia fluviatilis populatsiooni geneetiline analüüs
Gemmules were isolated from sponge specimens and stored at
4uC in mineral medium (M medium: 0.1 mM NaHCO3, Data analysis and the proportion model
0.05 mM Na2SiO3, 0.01 mM KCl, 0.1 mM MgSO4, 0.2 mM The data obtained by sequencing were analysed using the
CaCl2 [8]). In parallel,samples of sponge material were frozen in BioEdit program (http://www.mbio.ncsu.edu/bioedit/bioedit.
liquid nitrogen and stored at 220uC. html). Only sequencing data that displayed electropherograms of
Before starting, the culture gemmules were treated with 1% good quality were used.
hydrogen peroxide for 1 minute to minimize bacterial and fungal The absence or presence of notable intraindividual heteroge-
contamination [8]
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